ABSTRACT
Effect of isoniazid (INH) and its metabolites e.g. mono and diacetyl hydrazines (MAH and DAH respectively) was studied on circulating and tissue folates in mice (a species susceptible to INH tumorigenicity) and rats (a species resistant to INH carcinogenicity). It was observed that ip injection of INH, MAH and mydrazine sulfate (HS, 0.18 mg/g) decreased blood folates in mice while only HS and MAH decreased blood folates in rats. DAH had no effect on blood folates of mice or rats. Long term feeding of MAH and HS to mice decreased blood folates in treated mice at the age of 17 and 22 months respectively.
Subject(s)
Animals , Folic Acid/analysis , Hydrazines/pharmacology , Isoniazid/pharmacology , Liver/analysis , Lung/analysis , Mice , Mice, Inbred Strains , Mycobacterium/drug effects , Rats , Rats, Inbred StrainsABSTRACT
Patients suffering from pulmonary tuberculosis were investigated for the levels of isoniazid (INH) and its metabolites viz. acetyl-INH, mono and diacetyl hydrazines and ammonia. It was observed that 50% of the patients are slow inactivators of INH and almost all show an increase in circulating levels of NH3 at 6 hrs. Mono and diacetyl hydrazine levels in blood and urine were detectable in all the patients up+o 24 hrs though the maximum levels were observed at different intervals after the intake of INH.